Biotechnology

Download Assay Development: Fundamentals and Practices by Ge Wu PDF

By Ge Wu

Essential ideas and perform of assay development

The first entire, built-in therapy of the topic, Assay improvement: basics and Practices covers the necessities and strategies occupied with accomplishing an assay venture in both a biotechnology/drug discovery surroundings or a platform atmosphere.

Rather than trying finished assurance of all assay improvement applied sciences, the ebook introduces the main prevalent assay improvement applied sciences and illustrates the artwork of assay improvement via a number of normally encountered organic goals in assay improvement (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). simply enough organic heritage for those organic objectives is equipped in order that the reader can persist with the logics of assay improvement. Chapters discuss:

  • The fundamentals of assay improvement, together with foundational options and applications

  • Commonly used instrumental equipment for either biochemical assays and cell-based assays

  • Assay ideas for protein binding and enzymatic activity

  • Cell-based assays

  • High-throughput screening

An in-depth learn of the now well known Caliper's off-chip kinase assay offers an instructive, real-world instance of the assay improvement method.

Show description

Read Online or Download Assay Development: Fundamentals and Practices PDF

Similar biotechnology books

A Guide to Protein Isolation

This article takes the reader on a guided journey during the philosophical and actual foundations of protein isolation. aimed toward a pupil readership, it is going to even be very important to existence technology researchers confronted with the duty of setting apart a protein for the 1st time. The common sense of the final method of keeping apart a protein is defined and the actual rules of every separation strategy are made transparent by means of uncomplicated versions and analogies, drawn from daily reports.

Snapshots of Hemodynamics: An Aid for Clinical Research and Graduate Education

Hemodynamics give the opportunity to represent in a quantitative approach, the functionality of the guts and arterial approach, thereby generating information regarding which genetic and molecular strategies, and which abnormalities, are of value for knowing cardiovascular mechanisms in well-being and sickness. Snapshots of Hemodynamics: An relief for scientific examine and Graduate schooling, moment variation via Nico Westerhof, Nikos Stergiopulos and Mark I.

Opportunities for Biological Nitrogen Fixation in Rice and Other Non-Legumes: Papers presented at the Second Working Group Meeting of the Frontier Project on Nitrogen Fixation in Rice held at the National Institute for Biotechnology and Genetic Engineerin

Throughout the subsequent 30 years, farmers needs to produce 70% extra rice than the 550 hundreds of thousands plenty produced at the present time to feed the expanding inhabitants. Nitrogen (N) is the nutrient that the majority usually limits rice construction. At present degrees ofN use potency, we'll require at the least double the ten million lots of N fertilizer which are at the moment used every year for rice creation.

Extra info for Assay Development: Fundamentals and Practices

Example text

2006) Handbook of Assay Development in Drug Discovery. CRC, Boca Raton, FL. Ng, R. (2009) Drugs: From Discovery to Approval, 2nd ed. Wiley-Blackwell, Hoboken, NJ. Pollok, B. (2005) Assay development: An increasingly creative endeavour. Nat. Rev. Drug Discov. 4, 956–957. Prabhakar, U. and Kelley, M. ) (2008) Validation of Cell-Based Assays in the GLP Setting: A Practical Guide. Wiley, Hoboken, NJ. Pritchard, J. , et al. (2003) Making better drugs: Decision gates in non-clinical drug development.

Comb. Chem. High Throughput Screen. 10, 399–412. Bleicher, K. , and Alanine, A. I. (2003) Hit and lead generation: Beyond highthroughput screening. Nat. Rev. Drug Discov. 2, 369– 378. Burtis, C. , Ashwood, E. , and Bruns, D. E. (2007) Tietz Fundamentals of Clinical Chemistry, 6th ed. Saunders, New York. Chorghade, M. S. ) (2006) Drug Discovery and Development, Drug Discovery, Vol. 1. Wiley, Hoboken, NJ. Corey, M. J. (2009) Coupled Bioluminescent Assays: Methods, Evaluations, and Applications. Wiley, Hoboken, NJ.

In preclinical studies, a gram or more of the drug may be administered to one animal in a single does. Multiply this by repeated doses, the hundreds of animals in one study, and the numbers of different studies, several hundred grams or 14 CHAPTER 1 INTRODUCTION TO ASSAY DEVELOPMENT even several kilograms of the drug may be needed in the preclinical study. Thus, the substance used in preclinical testing may come from different batches of production. At this stage, the production group also constantly improves the process aimed at more efficient production processes and even larger production quantities for future clinical testing.

Download PDF sample

Rated 4.70 of 5 – based on 16 votes