By Ge Wu
Essential ideas and perform of assay development
The first entire, built-in therapy of the topic, Assay improvement: basics and Practices covers the necessities and strategies occupied with accomplishing an assay venture in both a biotechnology/drug discovery surroundings or a platform atmosphere.
Rather than trying finished assurance of all assay improvement applied sciences, the ebook introduces the main prevalent assay improvement applied sciences and illustrates the artwork of assay improvement via a number of normally encountered organic goals in assay improvement (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). simply enough organic heritage for those organic objectives is equipped in order that the reader can persist with the logics of assay improvement. Chapters discuss:
The fundamentals of assay improvement, together with foundational options and applications
Commonly used instrumental equipment for either biochemical assays and cell-based assays
Assay ideas for protein binding and enzymatic activity
An in-depth learn of the now well known Caliper's off-chip kinase assay offers an instructive, real-world instance of the assay improvement method.
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Extra info for Assay Development: Fundamentals and Practices
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Comb. Chem. High Throughput Screen. 10, 399–412. Bleicher, K. , and Alanine, A. I. (2003) Hit and lead generation: Beyond highthroughput screening. Nat. Rev. Drug Discov. 2, 369– 378. Burtis, C. , Ashwood, E. , and Bruns, D. E. (2007) Tietz Fundamentals of Clinical Chemistry, 6th ed. Saunders, New York. Chorghade, M. S. ) (2006) Drug Discovery and Development, Drug Discovery, Vol. 1. Wiley, Hoboken, NJ. Corey, M. J. (2009) Coupled Bioluminescent Assays: Methods, Evaluations, and Applications. Wiley, Hoboken, NJ.
In preclinical studies, a gram or more of the drug may be administered to one animal in a single does. Multiply this by repeated doses, the hundreds of animals in one study, and the numbers of different studies, several hundred grams or 14 CHAPTER 1 INTRODUCTION TO ASSAY DEVELOPMENT even several kilograms of the drug may be needed in the preclinical study. Thus, the substance used in preclinical testing may come from different batches of production. At this stage, the production group also constantly improves the process aimed at more efﬁcient production processes and even larger production quantities for future clinical testing.