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Additional resources for Autoradiography At the Cellular Level. Physical Techniques in Biological Research
For further details on autoradiographic differ entiation between various ^-emitters by a combined color-coupling and double-stripping film technique see Field et al. (1965). 3 Baserga (1961) and Baserga and Nemeroff (1962b) using thymidine- !! III. EXPOSURE 3 14 39 14 dZ-leucine- H thymidine- C and uridine- C tested different double-layer techniques with liquid emulsion and stripping film of which part of the gelatin layer was digested with trypsin. They found that the combination of 2 N T B liquid emulsion layers provided the most satisfactory results.
On the other hand, a further improvement of this method is important because of the many water-soluble substances the distribution of which within tissues and cells is of great interest. See also "Int. Conf. on High Resolution Autoradiography of Diffusible Sub stances" (1969). For a description of electron microscopic techniques in combination with autoradiography and some applications see Chapter 7. 3. Application of Different Isotopes I. Iodine-131 and Iodine-125 Radioactive iodine has been extensively used in studies of the iodine metabolism of the thyroid gland.
For instance, the length of the D N A synthesis period in a cell can be measured by application of 3 14 H-labeled thymidine first, and C-labeled thymidine second, after a certain time interval. Furthermore, with this technique it is possible to trace the metabolic behavior of two different substances in the same cell 14 simultaneously. By application of thymidine- C and simultaneous injec 3 tion of H-labeled amino acids or RNA precursors, protein or R N A metabolism can be studied in cells that are synthesizing D N A and in those that are not.